rabbit anti mouse rage  (Boster Bio)

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

Figure Lengend Snippet:

Article Snippet: Rabbit anti-mouse RAGE (1:100 dilution) , Boster , Cat# A03438; RRID: AB_3081636.

Techniques: Recombinant, Modification, Electron Microscopy, Software, Sterility

Journal: Translational Neuroscience

Article Title: Effects of enriched environment on the expression of β-amyloid and transport-related proteins LRP1 and RAGE in chronic sleep-deprived mice

doi: 10.1515/tnsci-2022-0301

Figure Lengend Snippet: Effect of EE on RAGE expression in chronic sleep-deprived mice. Note: (a) prefrontal cortex and hippocampal RAGE protein bands in each group; (b and c) analysis of grayscale values of prefrontal cortex and hippocampal RAGE/GAPDH in each group; (d) immunofluorescence staining of prefrontal cortex RAGE (green), CD31 (red), and DAPI (blue) in each group, magnification scale bar = 100 μm, other scale bar = 20 μm; (e) immunofluorescence staining of prefrontal cortex and hippocampal RAGE (red), CD31 (green), and DAPI (blue) in each group, magnification scale bar = 50 μm, other scale bar = 20 μm; (f) percentage of CD31-RAGE co-localization area of prefrontal cortex stretching CD31 area in each group; and (g) percentage of CD31-RAGE co-localization area of hippocampal stretching CD31 area in each group ( n = 3, **** P < 0.001).

Article Snippet: The following primary antibodies were used: Rabbit anti-mouse LRP1 primary antibody (ab92544, 1:1,000; Abcam), Rabbit anti-mouse RAGE primary antibody (ab3611, 1:1,000; Abcam), and Rabbit anti-mouse GAPDH primary antibody (AP0063, 1:5,000; Bioworld).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Nucleotide Sequence of the Specific Primers Used for Polymerase Chain Reaction Amplification

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Sequencing, Polymerase Chain Reaction

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Immunohistochemistry for HMGB1, TLR4, RAGE, and TNF-α in human cornea. Immunostaining of normal and Pseudomonas aeruginosa-infected corneas from human patients showed expression of HMBG1 (B), TLR4 (D), RAGE (F), and TNF-α (H) in corneas infected with P. aeruginosa when compared with similarly processed normal cornea (A, C, E, G). HMGB1, high mobility group box 1.

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Immunohistochemistry, Immunostaining, Infection, Expressing

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: RT-PCR and western blot for TLR4 and RAGE. Box A treatment significantly reduced corneal mRNA expression of TLR4 (A) and RAGE (B) at 5 days p.i. with no difference detected between groups in the uninfected, normal (N) cornea. Western blot and determination of relative protein expression by IDV analysis showed that Box A treatment reduced protein expression for TLR4 (C, E) and RAGE (D, F) in normal cornea and at 5 day p.i. No difference between treatment groups was seen at 3 days p.i. for either TLR4 or RAGE. An unpaired, 2-tailed Student's t-test determined significance for RT-PCR and protein expression data. P < 0.05 was considered significant; data are shown as mean ± SEM. IDV, integrated density value; RT-PCR, real-time polymerase chain reaction.

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of Ocular Pharmacology and Therapeutics

Article Title: HMGB1 Antagonist, Box A, Reduces TLR4, RAGE, and Inflammatory Cytokines in the Cornea of P. aeruginosa -Infected Mice

doi: 10.1089/jop.2018.0073

Figure Lengend Snippet: Immunohistochemistry for HMGB1 and TLR4 or RAGE. Dual immunostaining of infected corneas from Box A versus PBS-treated mice showed a modest pattern of co-labeling (yellow) of HMBG1 (red) with TLR4 (green) at 3 (A, B) and 5 (E, F) days p.i. only in the PBS-treated group. In contrast Box A treatment resulted in little to no colocalization at 3 (C,D) or 5 (G,H) days p.i. A similar pattern of co-localization (yellow) was seen for HMGB1 and RAGE at 3 days p.i. (I–L), but co-localization of HMGB1 and RAGE at 5 days p.i, was seen only in the PBS-treated corneas (M, N), but not in the Box A treated group (O,P). Magnification: 230 × (A, C, E, G, I, K, M, O), magnification: 460 × (B, D, F, H, J, L, N, P).

Article Snippet: After blocking for 1 h in 5% MTBST (TBS containing 0.05% Tween 20 and 5% nonfat milk), membranes were probed with primary antibodies: rabbit anti-mouse TLR4 (1:80; Abcam Cambridge, MA) and rabbit anti-mouse RAGE (1:1,000; Abcam) in 3% BSA TBST overnight at 4°C.

Techniques: Immunohistochemistry, Immunostaining, Infection, Labeling